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<p><b>OBJECTIVE</b>we aimed to investigate the mutation and expression of BRAF gene in mature T/NK cell lymphoma tissues and cell lines, explore the correlation between gene alterations and clinicopathological features and clinical outcomes of mature T/NK cell lymphoma.</p><p><b>METHODS</b>Firstly, we detected common mutant sites of BRAF (locus 1 799 mutation in exon 15 and loci 463, 465 and 468 mutation in exon 11) in lymphoma Jurkat, Hut-78 and YTS cell lines, normal peripheral blood lymphocytes, different types of mature T/NK cell lymphoma and reactive hyperplasia lymph nodes by direct sequencing. Then we measured the expression of BRAF in Jurkat, Hut-78, YTS cells and normal peripheral blood lymphocytes by real time-PCR and Western-blot detection. We also used immunohistochemistry (IHC) to detect the expression of BRAF in mature T/NK cell lymphoma tissues and reactive hyperplasia lymph nodes, and to analyze the correlation between the expression of BRAF and clinocopathological features and clinical outcomes.</p><p><b>RESULTS</b>We did not find common BRAF mutation in mature T/NK cell lymphoma tissues and cell lines, and the relatively expression of BRAF gene mRNA in normal peripheral blood lymphocytes, YTS, Hut-78 and Jurkat cells were 1.000, 5.207±0.013, 8.412±0.615 and 36.720±1.797, respectively, and protein expressions were 0.051±0.003, 0.102±0.013, 0.113±0.017 and 0.304±0.010, respectively, and the expression of BRAF in peripheral T cell lymphoma Jurkat cells was significantly higher than that of Hut-78, YTS cells and normal lymphocytes (P<0.05). Only 6 of 58 peripheral T cell lymphomas (10.3%) had positive BRAF expression, and were the subgroups of peripheral T cell lymphoma-unspecified type. The statistical data did not show any correlation between positive expression of BRAF and gender, age, clinical stage, location, lactate dehydrogenase in the 21 cases of peripheral T cell lymphoma-unspecified type (P<0.05), but the positive rate of BRAF in the effective treatment group (8.3%) was significantly lower than that of the invalid group (55.6%, P<0.05).</p><p><b>CONCLUSION</b>The expression of BRAF gene may become a marker of malignant biological characteristics and clinical therapeutic target of peripheral T cell lymphoma.</p>
Subject(s)
Humans , Exons , Immunohistochemistry , Killer Cells, Natural , Lymphoma, T-Cell, Peripheral , Genetics , Metabolism , Pathology , Proto-Oncogene Proteins B-raf , Genetics , Metabolism , Pseudolymphoma , Genetics , Metabolism , RNA, Messenger , MetabolismABSTRACT
Objective To encode the protein cystatin M with CST6 gene and construct a CST6-overexpression vector,and transfect it into the gastric cancer cells SGC-7901 in order to observe the effect of cystatin M on the proliferation and migration abilities of SGC-7901 cells.Methods There were three groups in the experiment:pcDNA3.1(+)-CST6 group,pcDNA3.1 (+)group and non-transfected group.RT-PCR and Western blot were used to identify the RNA expression of CST6 in SGC-7901/CST6 cells.The proliferation and migration abilities of the transfected cells were detected by MTT and cell wound healing assay,respectively.Results SGC-7901/CST6 cells stably expressed CST6 gene RNA and cystatin M protein.The proliferation and migration of pcDNA3 .1 (+)-CST6 group cells were reduced compared with those of cells in the other two groups (P<0.05).Conclusion Cystatin M can inhibit the proliferation and migration of SGC-7901 cells.
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Objective to study the characteristic typing of Helicobacter pylor(Hp)phenotypes and their sub-phenotypes in the patients with duodenal bulb ulcers(DU),and its clinical significance. Methods One hundred thirty-five cases with DU and 140 casses with chronic superficial gastritis were enrolled in this study. Determinations of serum cytotoxin-associated gene protein A (CagA),vacuolating cyto-toxin A(VacA),urease (Ure)A,UreB antibodies and their sub-phenotypes by immunoblotting were carried ou. Results Positive rate of middle-phenotypes of Hp infection in DU was significantly lower than that in chronic superficial gastritis (21.5%vs 27.9%,P<0.05).VacA and CagA antibodies might express alone. There had no significant difference among the expression rate of phenotype CagA, VacA antibodies and their sub-phenotype. But expression rate of Ure antibodies in Du was higher than that in chronic superficial gasstritis (P<0.05).In infection of Hp type I, the expression rate of sub-phenotypes 30ku UreA in DU was hronic superficial gastritis (P<0.05). Conclusions The VacA is not for expressing higher than that inpression of Hp exists many sub-phenotypes ( 128 ku CagA 116 ku CagA, 95 ku VacA, 91 ku CagA 0 ku UreA),and it probably causes formation of DU by comprehensive effect. Hp type I with sub phenotype expressing 30 ku Urea may be more pathogenic in DU formation.
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Objective To observe the effect of Silbinin Capsuon serum interleukin-8 and tumor necrosis factor-?in NAFLD patients.Methods 58 patients with NAFLD were randomized into two groups.The treatment group including 29 cases received 70mg of Silbinin Capsules each time,three times a day.The control group including 29 cases received 100mg of goucurolactone tablets each time,three times a day.At the same time,both groups control food and drink and proper physical exercises.One course of treatment was 30 days in the study.Observe the changes of serum ALT,AST,IL-8 and TNF-?before and after treatment.Results The serum level of ALT,AST,IL-8 and TNF-?were improved significantly in both groups after treatment(P
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OBJECTIVE: To discuss the the control of hyperglycemia in patients with severe acute pancreatitis(SAP).METHODS: Patients were treated with NPH(subcutaneous injection) or continuous subcutaneous insulin injection(CSII) or Insulin glargine(subcutaneous injection).Incidences of hypoglycemia and hyperglycemia and the blood sugar level within 5 days of admission were measured.RESULTS: The incidences of blood glucose level being ≤4.0 mmol?L-1 or ≥11.1 mmol?L-1 were significant different in CSII group and insulin glargine group compared with NPH group respectively(P